Flow cytometric analysis showing the binding in vitro of recombinant mouse E-selectin chimera to neutrophils from C57Bl/6 and PSGL-1^-/- mice. (A) Binding to neutrophils of untreated C57Bl/6 and PSGL-1^-/- mice along with cells alone as controls. (B) Binding to C57Bl/6 neutrophils and C57Bl/6 neutrophils after 3 hours of TNF-α local injection (intrascrotal). (C) Binding to C57Bl/6 neutrophils and C57Bl/6 neutrophils after 3 hours of TNF-α injection (intraperitoneal). (D) Binding to PSGL-1^-/- neutrophils and PSGL-1^-/- neutrophils after 3 hours of TNF-α systemic injection (intraperitoneal). Binding was detected with PE. The data are representative of 4 experiments.