![Figure 1. Analysis of morphology and αIIb-β3 expression of normal MKs. (A) Cytospins were observed at light microscopy after May-Grunwald staining (× 20 magnification, 0.50 aperture objective lenses [Carl Zeiss, Oberkochen, Germany]). Note the presence of cells belonging to various maturational stages of megakaryocytopoiesis. (B) αIIb-β3 expression (white area) of normal MK cells was examined with an anti-CD41a MoAb directly conjugated to phycoerythrin, and fluorescence was analyzed by flow cytometry. Cells treated with an isotype-matched irrelevant MoAb directly conjugated to phycoerythrin represent the negative control (black area). The x-axis indicates fluorescence intensity; the y-axis, relative number of cells. (C) Morphologic analysis of purified MKs. May-Grunwald staining (× 40 magnification, 1.0 aperture objective lenses [Carl Zeiss]). Note the presence of small megakaryoblasts near mature megakaryocytes. Morphology was evaluated with an Ultraphot universal photomicroscope (Carl Zeiss, Oberkochen, Germany). The camera was inserted within the microscope. No software was used to optimize the figure. Objective lenses 20 × and 40 ×.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/10/10.1182_blood-2003-07-2597/6/zh80220469300001.jpeg?Expires=1722712297&Signature=CmAOVgYaLD~vxEEZDk7wNZZnLjxUYC8nb2TUWfYClKehhlMcqLrkWOTWBwbQEsWRH4DynmWRmGmHVbI9TOQPEYh7HPVUEldp1UJhGQGnf-zltgpwkLv7yeuWf4VPGrzFdnNgfXTTE1Ne252nyo2TbC4qu4Olc1JTDCwdXkM6xXHQxgHJaYu7A12AN3YEwJJslaWvXdpwVAI82ZAruxjN8NCfcuQOOMVUDh6Adr0JNptUJpzsRsxUb0BEDfKd~LkEw1j2tZNzrJvQW~SqmtLT4Pdbb9-XtWFY~atGAlycj4nqinFdprW5cvwd9K22Dx4Nm~qM7GHtM7DYrsa4Q2LMQw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Analysis of morphology and αIIb-β3 expression of normal MKs. (A) Cytospins were observed at light microscopy after May-Grunwald staining (× 20 magnification, 0.50 aperture objective lenses [Carl Zeiss, Oberkochen, Germany]). Note the presence of cells belonging to various maturational stages of megakaryocytopoiesis. (B) αIIb-β3 expression (white area) of normal MK cells was examined with an anti-CD41a MoAb directly conjugated to phycoerythrin, and fluorescence was analyzed by flow cytometry. Cells treated with an isotype-matched irrelevant MoAb directly conjugated to phycoerythrin represent the negative control (black area). The x-axis indicates fluorescence intensity; the y-axis, relative number of cells. (C) Morphologic analysis of purified MKs. May-Grunwald staining (× 40 magnification, 1.0 aperture objective lenses [Carl Zeiss]). Note the presence of small megakaryoblasts near mature megakaryocytes. Morphology was evaluated with an Ultraphot universal photomicroscope (Carl Zeiss, Oberkochen, Germany). The camera was inserted within the microscope. No software was used to optimize the figure. Objective lenses 20 × and 40 ×.
