Figure 1.
Figure 1. Generation of a targeted disruption in the mouse PF4 gene. (A) Strategy for creating a mPF4 knock-out mouse. E = EcoRI, X = XhoI, neo = neomycin resistance gene, and TK = thymidine kinase gene. The numbered arrows refer to the primers used to check for a targeted event by genomic PCR. (B) Southern blot of genomic DNA from ES cells with 1 correctly targeted line (+/–) and 2 wild-type lines (+/+) after a combined EcoRI/XhoI digest and using the genomic probe shown in panel A. (C) Genomic PCR demonstrating correct targeting of the mPF4 gene in the targeted ES line (+/–) and not in the wild-type ES cells (+/+). (D) Southern blot of genomic DNA from mPF4+/+ and mPF4+/– and mPF4–/– mice for the targeted disruption of the mPF4 gene following a EcoRI/XhoI double digestion and using the genomic probe shown in panel A.

Generation of a targeted disruption in the mouse PF4 gene. (A) Strategy for creating a mPF4 knock-out mouse. E = EcoRI, X = XhoI, neo = neomycin resistance gene, and TK = thymidine kinase gene. The numbered arrows refer to the primers used to check for a targeted event by genomic PCR. (B) Southern blot of genomic DNA from ES cells with 1 correctly targeted line (+/–) and 2 wild-type lines (+/+) after a combined EcoRI/XhoI digest and using the genomic probe shown in panel A. (C) Genomic PCR demonstrating correct targeting of the mPF4 gene in the targeted ES line (+/–) and not in the wild-type ES cells (+/+). (D) Southern blot of genomic DNA from mPF4+/+ and mPF4+/– and mPF4–/– mice for the targeted disruption of the mPF4 gene following a EcoRI/XhoI double digestion and using the genomic probe shown in panel A.

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