Oct2 and BOB.1/OBF.1 overexpression potentiates 5-aza-dC-induced Ig transcription in cHL cells. L428-neo or L428-Oct2 cells were pretreated for 24 hours with 1 μM 5-aza-dC. After an additional 24 hours in normal growth medium, cells were nucleofected with the BOB.1/OBF.1-expressing vector pCFG5-BOB.1 or by the empty vector pCFG5-IEGZ. On the next day cells were harvested and used for RNA or protein analysis as described in “Materials and methods.” (A) Expression of Oct2, BOB.1/OBF.1, and IgG gamma chains in cell lysates were assessed by Western blots. (B) RT-PCR analysis of Ig transcription was done as described in the legend for Figure 5 and in “Materials and methods.”