Figure 5.
![Figure 5. Activity of T16 depends on the external choline concentration. Effect of external choline concentration (0.04 mM, 0.123 mM, 0.37 mM, 1.1 mM, 3.3 mM, and 10 mM) on the uptake of [3H]-T16 (50 nM) and the antimalarial IC50 of T16. Uptake of T16 was measured over 10 minutes at 22°C. Under these conditions, T16 uptake is in its linear initial phase (data not shown). Uptake experiments and sensitivity assays used intact infected erythrocytes. Uptake data are mean ± SE for duplicate observations from 3 individual experiments. Sensitivity assay data are mean ± SE for triplicate observations from 3 individual experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/10/10.1182_blood-2004-03-1084/6/zh80220469140005.jpeg?Expires=1725312569&Signature=benW4VNsp5vW9RuG4wHJi2p4P15~dfX~Z0U6t0oOYQa6FfOks8D3KZBWsl0KIwLyD4LnldzL5MwlB5KH9YFBpG0uUvEO1PxqWSwxZ2ewXHZHCg280oQPRh80ip97HBffirLjDURxCRpi7LrOUQKZFTbdq2GZ-fnKD8CQh14VPUS7Z8zMmWUjXkTp1PTBG1LSCvkN45F-p6inlg3mLXtVlOe0m6bl~PrhpCnQHF2T2Tfg31u8Ij7049O-0BxrNWGHZTBBm13kdPmH2sXqr97VMp5bKP91n3VsSO8qtd1~170JFPtJhBKNqP70UpYNFmCkuYQEgEsnHq51GLEjFu4oeQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activity of T16 depends on the external choline concentration. Effect of external choline concentration (0.04 mM, 0.123 mM, 0.37 mM, 1.1 mM, 3.3 mM, and 10 mM) on the uptake of [3H]-T16 (50 nM) and the antimalarial IC50 of T16. Uptake of T16 was measured over 10 minutes at 22°C. Under these conditions, T16 uptake is in its linear initial phase (data not shown). Uptake experiments and sensitivity assays used intact infected erythrocytes. Uptake data are mean ± SE for duplicate observations from 3 individual experiments. Sensitivity assay data are mean ± SE for triplicate observations from 3 individual experiments.
