Acetylation profile of histone H3 in the mGata1 locus. At the top of the figure, the mGata1 locus is shown. Genes are depicted as black boxes and, below that, the positions of the hematopoietic DNase I HSs (gray arrows) and widely expressed DNase I HSs (black arrows) are marked. Panels below the locus show the profile of acetylated H3 at different points in the mGata1 locus as determined by ChiP using real-time Taqman PCR analysis in an erythroid cell line (MEL), megakaryocytic cell line (L8057), primary eosinophils (Io eosinophils), and a fibroblast cell line (NIH3T3 cells). The fold enrichment of acetylated H3 (y-axis) is shown at different points along the mGata1 locus (x-axis). The coordinates are in kilobases with respect to the transcriptional start site of the GATA1 IE promoter. The black bars show average fold enrichment results from duplicate real-time PCR reactions performed on 3 independent ChiP preparations. Error bars (1 SD) are shown. We were not able to assess histone acetylation status of chromatin overlying mHS–57.5 (note that the closest primer pair to mHS–57.5 is ∼ 1.0 kb away), as sequence encompassing mHS–57.5 is repetitive.
