Membrane-bound hemichromes, autologous IgG, and complement C3c fragment; aggregated band 3 and phagocytosis in trophozoite-parasitized normal and mutant RBCs. Parameters were measured in trophozoite-parasitized normal RBCs (□) and trophozoite-parasitized mutant RBCs (▪)in HbAS (A) and beta-thal trait (B). Hemichromes are expressed in nmol/mL membranes; aggregated band 3, as percentage aggregated band 3 over total band 3; membrane-bound autologous IgG and C3c, as milliabsorbance units/min/107 RBCs; and phagocytosis, as number of phagocytosed RBCs per monocyte. Data are mean values ± SD (vertical bars). Numbers of separate experiments each performed with a different donor were as follows: controls, 8; HbAS, 4; and beta-thal trait, 4. Significance of differences between normal and mutant trophozoites was assessed by t test for paired samples. ^**P < .01; and no asterisk, P > .05.