Figure 1.
Dual immunoglobulin-isotype protein expression on HCs. For dual isotype expression, HCs were identified as hiFSC/hiSSC/hiCD20 PBMCs with the use of PerCP anti-CD20. HCs were then analyzed for the surface coexpression of IgM and IgD or IgM and IgG with the use of PE-F(ab′)2 anti-IgM monoclonal antibody (MoAb) together with FITC-F(ab′)2 anti-IgD or FITC-F(ab′)2 anti-IgG MoAbs. Top row shows contour plots for case 83; bottom row, for case 93. For each case, HCs stained with PE- and FITC-isotype controls (left contour plot), with anti-IgM and anti-IgD (center), or with anti-IgM and anti-IgG (right) are represented. On the top-right corner of each plot, the percentage of dual immunoglobulin-positive HCs is indicated.
