HLA-G5 localizes in the erythroid cells in AGM from a 32-day embryo. HES coloration of a 32-day-paraffin-embedded embryo section (left) permits the localization of the AGM (arrow) for which higher magnifications of the same AGM region stained with antibodies to HLA-G5/-G6, CD71, CD34, and CD45 are represented (right panel). HLA-G5 staining was examined regarding the CD71-expressing erythroid cells that flow in the aorta, the CD34-positive endothelial cells lining the aorta, and the CD34- and CD45-positive pluripotent stem cells which form a cluster associated to the ventral wall of the dorsal aorta. The arrows point to the few residual pluripotent stem cells in the sections stained with the anti-CD71 or anti-CD45 mAb. Left panel is shown at× 100, and right panels at × 600.