Single-cell analysis for granzymes A and B demonstrates discordant expression in activated human PBMC subsets. (A) Representative FACS analysis for intracellular granzyme A and B expression in unactivated and activated (ConA/IL-2 for 5 days) healthy human PBMCs. Lineage-specific (CD4, CD8, and CD56) and nonlineage (CD25) surface markers are shown. (B) Representative FACS analysis for granzyme A and B expression in resting CD56⁺CD8⁺ T-cell and CD56⁺CD8^- NK cell subsets from 2 different donors. Data are representative of 6 or more healthy donor samples with similar expression patterns. Percentages of total cells in each quadrant are indicated in the upper right corner of each subpanel.