Amplification of cDNA from the propositus and a healthy control. cDNA from platelet RNA was amplified from the propositus and a healthy control. (A) Amplification of exons 20 to 28 resulted in one product of 984 bp in the healthy control (lane 2); however, 3 bands (984 bp, 825 bp, and 684 bp) were observed in the patient's amplified product (lane 1), corresponding to the correct product, and products missing exon 26 and exons 23/26, respectively. The mRNA corresponding to the remaining mature VWF peptide was also amplified in different segments; however, no difference was observed between the healthy control and the patient. (B) Amplification of exons 16 to 21 generated a 705-bp product (lane 1, H2O; lane 2, healthy control cDNA; and lane 3, the patient's cDNA). (C) Amplification of exons 28 to 31 generated a 1371-bp fragment (lane 4, H2O; lane 5, healthy control; and lane 6, patient), and amplification of exons 30 to 36 produced a 1103-bp fragment (lane 1, H2O; lane 2, healthy control; and lane 3, patient). (D) Amplification of exons 35 to 41 generated a 1216-bp fragment (lane 4, H2O; lane 5, healthy control; and lane 6, patient), and amplification of exons 40 to 52 produced a 1620-bp fragment (lane 1, H2O; lane 2, healthy control; and lane 3, patient).
