Surface α1-3 fucosylation of CB mononuclear cells enhances engraftment in bone marrows of sublethally irradiated NOD/SCID mice. Sham-treated or FTVI-treated CB mononuclear cells were injected intravenously into sublethally irradiated NOD/SCID mice. Control mice were injected only with saline. After 6 weeks, the mice were killed and hematopoietic cells isolated from bone marrow and peripheral blood were analyzed for engraftment of human-derived hematopoietic cells. (A) The number of human clonogenic hematopoietic progenitor cells in bone marrow was determined. (B) Bone marrow (BM) and peripheral blood (PB) cells were incubated with a FITC-conjugated mAb to human CD45 or with a FITC-conjugated isotope-matched control mAb and analyzed by flow cytometry. The percentage of cells that expressed human CD45 is shown. The data represent the mean ± SD of measurements from 5 mice in each experimental group.