Figure 4.
Figure 4. Surface α1-3 fucosylation of CB CD34+ cells augments rolling on P-selectin and E-selectin under flow. (A) Sham-treated or FTVI-treated CB CD34+ cells were perfused over immobilized human P-selectin or over a control surface coated only with HSA in the presence or absence of a blocking mAb to P-selectin (G1) or PSGL-1 (PL1). (B) Sham-treated or FTVI-treated CB CD34+ cells were perfused over immobilized human E-selectin or over a control surface coated only with HSA in the presence or absence of a blocking mAb to E-selectin (ES1) or PSGL-1 (PL1). The wall shear stress was 0.5 dyne/cm2. After 4 minutes, the number of rolling cells was quantified. The data represent the mean ± SEM of 3 independent experiments.

Surface α1-3 fucosylation of CB CD34+ cells augments rolling on P-selectin and E-selectin under flow. (A) Sham-treated or FTVI-treated CB CD34+ cells were perfused over immobilized human P-selectin or over a control surface coated only with HSA in the presence or absence of a blocking mAb to P-selectin (G1) or PSGL-1 (PL1). (B) Sham-treated or FTVI-treated CB CD34+ cells were perfused over immobilized human E-selectin or over a control surface coated only with HSA in the presence or absence of a blocking mAb to E-selectin (ES1) or PSGL-1 (PL1). The wall shear stress was 0.5 dyne/cm2. After 4 minutes, the number of rolling cells was quantified. The data represent the mean ± SEM of 3 independent experiments.

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