Surface α1-3 fucosylation of CB CD34⁺ cells with exogenous FTVI and GDP fucose increases sLe^x epitopes. CB CD34⁺ cells were incubated in the presence or absence of FTVI and GDP fucose. The cells were then incubated with PE-conjugated anti-CD34 mAb and with anti-sLe^x mAb HECA-452 identified with a FITC-conjugated second antibody. Binding was measured by flow cytometry. Cells incubated with isotype-matched control antibodies were used to determine fluorescence thresholds for specific binding. The results are representative of 5 independent experiments. The percentage of cells in each quadrant is indicated.