Figure 6.
Effect of cycloheximide on LIR7-mediated IL-4 generation. Highly purified basophils were either incubated with IL-3 (A) or not (B), incubated with 0.1 μg/mL mAb to LIR7 or control IgG1, and activated by addition of goat anti-mouse IgG for 4 hours in the absence (□) or presence of 1 μM (▨) or 10 μM (▪) cycloheximide. Goat anti-mouse IgG was cleared from the supernatants and IL-4 was assayed by EIA. A single representative experiment of 2 is depicted. CXH indicates cycloheximide.
