Figure 1.
![Figure 1. GPI-anchor-deficient phenotype of alveolar macrophages and dendritic cells. (A) Alveolar macrophages (AM; left) or dendritic cells (DC; middle) cultured for 6 weeks were stained with mAb anti-HSA. Peripheral blood leukocytes gated for lymphocytes (PBL) were stained with mAb anti-CD48 (right), which is expressed on both T and B lymphocytes. Shown are fluorescence intensity plots of cells from littermate control (thin dotted lines) or LysMCre/Pig-aflox mice (bold lines), and antibody controls (dashed lines). (B-C) Responses to LPS. Control (wild type [WT]; •) or GPI-anchor-deficient (knock out [KO]; ○) AMs (B) or DCs (C) were stimulated with LPS; mean concentrations of TNF-α released of at least 3 independent experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/9/10.1182_blood-2004-02-0671/6/zh80210468490001.jpeg?Expires=1723911856&Signature=Cft4v3oBfoPXitEwByYaiju6rwruu0uFGOdagQGp7mZhEQGJI14-gRueZRX3dKHykhnWTSttsUovrxsOU0a456M4fiKjZo4CwtU0QPh-C~dEcknzMSxBTPaZKSjXLZm~Tjvr4Qp0~FT6KQTb0yWn-8tAkPUyN9wUEHSgteZEoiUBmTPbOHSDTJfTMZ3nNQA29W7h0A2fwl3v7UHlI~3OnyzjgmLlsckTX4fFUwiWMLUkKTCkO1dlyYGGtzvoIJU7vTIgtt7dfydH61PfcHMauKQkhwMx~~pKKMQzqKfgFz0t-U1eCKoQcp0ZivALd1XrSDJdGUz1TZMZpH5FK43rJQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GPI-anchor-deficient phenotype of alveolar macrophages and dendritic cells. (A) Alveolar macrophages (AM; left) or dendritic cells (DC; middle) cultured for 6 weeks were stained with mAb anti-HSA. Peripheral blood leukocytes gated for lymphocytes (PBL) were stained with mAb anti-CD48 (right), which is expressed on both T and B lymphocytes. Shown are fluorescence intensity plots of cells from littermate control (thin dotted lines) or LysMCre/Pig-aflox mice (bold lines), and antibody controls (dashed lines). (B-C) Responses to LPS. Control (wild type [WT]; •) or GPI-anchor-deficient (knock out [KO]; ○) AMs (B) or DCs (C) were stimulated with LPS; mean concentrations of TNF-α released of at least 3 independent experiments are shown.
