Adipogenic potential of CR cells and BM cells. Passage 2 cells collected from 6 donors were plated at 100 cells per 60-cm² dish and cultured for 14 days. The cells were then incubated in adipogenic medium for an additional 21 days. (A) Adipocyte colonies stained with Oil Red-O were shown as red colonies. The same dishes were then stained with Crystal Violet and the total number of colonies was determined. Representative pictures are shown. (B) The ratio of Oil Red-O-positive colonies to the total number of colonies was calculated for each dish, and Oil Red-O-positive colony efficiency is shown for young and elderly donors. The data are expressed as mean ± SD (n = 3).