Platelet accumulation in developing thrombi in wild-type and low TF mice. Platelets were labeled in developing thrombi with Alexa 660–conjugated chicken antirat antibody bound to rat anti-CD41 antibody. Each curve, as labeled, is the median integrated platelet fluorescence intensity for multiple thrombi of each genotype: WT (23 thrombi in a total of 2 mice) and LTF (18 thrombi in 3 mice). The time points of the early phase, plateau phase, and peak are indicated. Fluorescence of platelets in arbitrary units is presented as a function of time.