American Society of Hematology

Figure 1.

$Figure 1. Purification strategy for isolating cells stained with CD34/CD10 or CD19. ALL blast cells were gated on the basis of low forward and side scatter (A) and subsequently gated for expression of CD34-PE and CD10- or CD19-FITC, R1 to R4 (B). Sort gates R1 to R4 were separated by at least 10 channels, and quadrants K1, K2, and K4 in panel B were set up to exclude at least 99.9% of cells in isotype controls. (C-D) Reanalysis of CD34+/CD19+ (C) and the CD34+/CD19- subfractions (D) after sorting.$

Purification strategy for isolating cells stained with CD34/CD10 or CD19. ALL blast cells were gated on the basis of low forward and side scatter (A) and subsequently gated for expression of CD34-PE and CD10- or CD19-FITC, R1 to R4 (B). Sort gates R1 to R4 were separated by at least 10 channels, and quadrants K1, K2, and K4 in panel B were set up to exclude at least 99.9% of cells in isotype controls. (C-D) Reanalysis of CD34⁺/CD19⁺ (C) and the CD34⁺/CD19^- subfractions (D) after sorting.

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