Phosphorylation of NFκB, STAT5, and STAT6 in response to KL and antigen. Cultures were incubated overnight in the absence (Deprived Cells) or presence (Nondeprived Cells) of IL-6 and KL and presence of antigen-specific IgE. Deprived cells were stimulated for 15 or 60 seconds with 100 ng/mL KL, antigen (Ag), or both stimulants as indicated (A). Nondeprived cells were stimulated with antigen for 60 seconds (B). Immunoblots were probed with antibodies against the activated phosphorylated forms of NFκB, STAT5, and STAT6 (designated with the prefix “p-”). Blots were also probed for the tyrosine kinase Syk or Kit to verify uniformity of loading. The blots are representative of 3 experiments.