Time course of phosphorylation of MAP kinases and Akt in response to antigen. Cells were incubated overnight with antigen-specific IgE, IL-6, and KL (nondeprived cells). Cells were then stimulated with 100 ng/mL antigen for the indicated periods of time as described for Figure 4. Immunoblots were quantitated bydensitometric scanning. Similar results were obtained in a second experiment.