Figure 3.
![Figure 3. Antigen but not KL induces translocation of PKC. Human mast cells were incubated overnight in the presence of IgE and in the absence of KL and IL-6, as described in the legend for Figure 1, and then either not stimulated or stimulated with 100 ng/mL antigen (Ag), KL, or both stimulants for 5 minutes. Immunoblots were prepared from the membrane fraction of whole-cell lysates using antibodies against the indicated isoforms of PKC, phosphorylated PKCα/β [p-PKC (α+β)], or phosphorylated pan-PKC [p-PKC (pan)]. The photographs were overexposed to maximize detection of membrane bound PKC in KL-stimulated cells. Identical results were obtained in 2 other experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/8/10.1182_blood-2004-02-0631/6/zh80200467680003.jpeg?Expires=1724962587&Signature=08~r-m2vMC4Jr9dGOUXwjCO4OQbc-ky4op1gU449pAmnJo9EsDMxXAtUmJfSREcLxjujhO-zms4KVNtq0Nm60K1BfOR51BWa99YrGokg0l3vJ316~XN-fN2Zp8jWk2isBEt8CfKorqwPlwbwnGG4ZJpokCN3E0iP-lTJstvk9ltn3yl6I3MudDHFgcp6LBIXSvjA5~oRSIhte4VUVbvl1EMeRsOAe-A4p9FIzzg3bEfJb59xqMn4L~gFs97euFjrc2XM-kjnZlOUoKkN0ypMPx8zATZoN-u3H1iWnVAmpCaENbKaTU0X5WfYGnOw5IAdxHQHPQEREcDalp~Fh2TV9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Antigen but not KL induces translocation of PKC. Human mast cells were incubated overnight in the presence of IgE and in the absence of KL and IL-6, as described in the legend for Figure 1, and then either not stimulated or stimulated with 100 ng/mL antigen (Ag), KL, or both stimulants for 5 minutes. Immunoblots were prepared from the membrane fraction of whole-cell lysates using antibodies against the indicated isoforms of PKC, phosphorylated PKCα/β [p-PKC (α+β)], or phosphorylated pan-PKC [p-PKC (pan)]. The photographs were overexposed to maximize detection of membrane bound PKC in KL-stimulated cells. Identical results were obtained in 2 other experiments.
