American Society of Hematology

Figure 3.

$Figure 3. Calibration of density gradients to MCHC. RBCs were treated with nystatin to give various MCHCs and then analyzed on a 7-density gradient. (A) Density distributions of SS RBCs with measured MCHC = 328 g/L (32.8 g/dL) after nystatin treatment; MCHC calculated from the density score using the calibration curve in panel B was 326 g/L (32.6 g/dL). (B) Calibration curve. For a panel of nystatin-treated cells with various MCHC, the calculated density scores were plotted versus MCHC measured prior to centrifugation (▵). Alternatively, an unfractionated sample was centrifuged, individual gradient fractions harvested, and MCHC measured (▪); these samples were assigned a density score according to their position on the gradient (eg, fraction 2 DS = 200). (C) Reticulocyte density distribution for the SS RBCs sample in panel A. SS reticulocyte MCHC = 308 g/L (30.8 g/dL) calculated from DS using the calibration curve.$

Calibration of density gradients to MCHC. RBCs were treated with nystatin to give various MCHCs and then analyzed on a 7-density gradient. (A) Density distributions of SS RBCs with measured MCHC = 328 g/L (32.8 g/dL) after nystatin treatment; MCHC calculated from the density score using the calibration curve in panel B was 326 g/L (32.6 g/dL). (B) Calibration curve. For a panel of nystatin-treated cells with various MCHC, the calculated density scores were plotted versus MCHC measured prior to centrifugation (▵). Alternatively, an unfractionated sample was centrifuged, individual gradient fractions harvested, and MCHC measured (▪); these samples were assigned a density score according to their position on the gradient (eg, fraction 2 DS = 200). (C) Reticulocyte density distribution for the SS RBCs sample in panel A. SS reticulocyte MCHC = 308 g/L (30.8 g/dL) calculated from DS using the calibration curve.

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