Effect of neutralizing anti–human-TGFβ antibodies on anti-inflammatory activity of ectosomes. HMDMs were incubated with medium alone (Ø), medium with ectosomes, or medium supplemented with zymosan (5 μg/mL) for 24 hours. Alternatively, zymosan medium was supplemented with ectosomes alone (no antibodies) or ectosomes with 5 μg/mL or 50 μg/mL αTGFβ-Abs. Supernatants were analyzed for IL-8, IL-10, and TNFα.