Figure 1.
Determination of bacterial concentrations in the semiautomated killing assay and comparison with conventional plating. Serial dilution of the E coli suspension was prepared, and bacteria were grown on a 96-well plate as described in “Materials and methods.” (A) Changes in absorption at 650 nm were followed in time. (B) Initial concentration of bacteria was plotted against the obtained incubation time values (tinc). Error bars represent SD of 4 parallel samples. Fitting a straight line on the experimental data resulted in the following calibration equation: E coli = \(2.2206{\times}\mathrm{e}^{-0.0365{\times}\mathrm{t}}_{\mathrm{inc}}{\times}10^{6}{/}\mathrm{mL}\)
. The results of 1 representative experiment out of 29 similar ones are shown. (C) Time course of the decrease in surviving bacteria followed by the semiautomated assay. (D) Time course of the decrease in surviving bacteria determined by conventional plating. Where indicated, cytochalasin B (CB) was present at a concentration of 10 μM. The results shown are representative of 4 similar experiments, and error bars represent standard deviation of 4 parallel samples.
![Figure 1. Determination of bacterial concentrations in the semiautomated killing assay and comparison with conventional plating. Serial dilution of the E coli suspension was prepared, and bacteria were grown on a 96-well plate as described in “Materials and methods.” (A) Changes in absorption at 650 nm were followed in time. (B) Initial concentration of bacteria was plotted against the obtained incubation time values (tinc). Error bars represent SD of 4 parallel samples. Fitting a straight line on the experimental data resulted in the following calibration equation: E coli = \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(2.2206{\times}\mathrm{e}^{-0.0365{\times}\mathrm{t}}_{\mathrm{inc}}{\times}10^{6}{/}\mathrm{mL}\) \end{document}. The results of 1 representative experiment out of 29 similar ones are shown. (C) Time course of the decrease in surviving bacteria followed by the semiautomated assay. (D) Time course of the decrease in surviving bacteria determined by conventional plating. Where indicated, cytochalasin B (CB) was present at a concentration of 10 μM. The results shown are representative of 4 similar experiments, and error bars represent standard deviation of 4 parallel samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/9/10.1182_blood-2004-03-1005/6/zh80210468710001.jpeg?Expires=1725025652&Signature=CE7HQDM0KB49jgqSC9JxN5D01oO56Opt2pVK3QXHQfLJwYckV1-1SoLcnQ2DUcsadHxG6pPnzrp0LBHwSv6c2avihQh9Go9AocZO6puCupA-HUtAmAYNLimiewfqMTxPfmHCEtHcSKckdzle2pngJ751aTZrpObYv5dhu0fh92C1XD7cyTirpXz9roIQEBHy9gtNQiT9ef1XpLMwRweMhA44clGXusuC75~TICtA~t9B6UEgm8SVYzdoRfYiAXPbIgKAQ6m5mbTbMa1xsJrIblPjNtVGMC8DMYQ3JCll0GTtY6O~D8i7lteKZ920TV47Aii8gq86oePdamhu7L4nfQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
