Analysis of TCRVβ by spectratype. RNA was extracted from sorted CD62L⁺ (□) and CD62L^– (▪) T cells from donors 1 and 5 immediately following isolation. (A) Spectratype analysis was performed on 23 Vβ families where 3 representative families are shown for donor 1 and 5 for CD62L⁺ and CD62L^– T cells. (B) TCR complexity was assessed by counting the number of peaks per Vβ family for CD62L⁺-sorted and CD62L^–-sorted T cells.