Figure 4.
![Figure 4. Transduction of a c-kit+ cell line is inhibited by soluble SCF. (A) Transduction of MO7e cells, as shown in Figure 3, but performed in the presence [ampho (S) and SCF-eco (S)] or absence (SCF-eco) of 100 ng/mL human recombinant soluble SCF. Data are mean values from 2 independent transductions. (B) PCR analysis of DNA isolated from transduced cells shown in panel A. Lanes 2 to 4 show transductions performed in the presence of soluble SCF. (Top) PCR amplification using EGFP primers. (Bottom) PCR amplification of the same DNA samples using IFN-α primers. Lane 1, the 100-base-pair ladder; lane 2/a, amphotropic virus; lane 3/m, mock-transduced cells; lane 4/S-e, SCF-eco virus; and lane 5/S-e, SCF-eco virus.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/9/10.1182_blood-2003-10-3717/6/zh80210468400004.jpeg?Expires=1724193929&Signature=3Rn3Bvyym7LNYzNXSx6MtJugYayrYvGSV18jEzUi23dIclH-HnEPgA3JNb-BYwE-eI6fpXZfWxy81bmT9Tq7dKzIJyDU5Aj4znG1uP01WnWMIcdh-LqxCeDs64A~72oQZ0Hr~txqrtSiZD20kvzl7PpKbpEonfw2LfWcDVmduXpKdFrlT51numqJnYnvYIXNunnCdGAB-MuIGXJjlAM7hCLd2qlAIHrnTmpM9z28svV4wcJXm3k7h0B-k5QPZyIhIqHC73OJ-vsJAqKt5HLoPKBFgblR9~ZTR27yW3Ab2YGKaotq4Pjo-2KKC6uVPzT2r3KPjEZv5mDFX47nOPMfgg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transduction of a c-kit+ cell line is inhibited by soluble SCF. (A) Transduction of MO7e cells, as shown in Figure 3, but performed in the presence [ampho (S) and SCF-eco (S)] or absence (SCF-eco) of 100 ng/mL human recombinant soluble SCF. Data are mean values from 2 independent transductions. (B) PCR analysis of DNA isolated from transduced cells shown in panel A. Lanes 2 to 4 show transductions performed in the presence of soluble SCF. (Top) PCR amplification using EGFP primers. (Bottom) PCR amplification of the same DNA samples using IFN-α primers. Lane 1, the 100-base-pair ladder; lane 2/a, amphotropic virus; lane 3/m, mock-transduced cells; lane 4/S-e, SCF-eco virus; and lane 5/S-e, SCF-eco virus.
