Figure 3.
AP23464 inhibits tyrosine phosphorylation of isolated wild-type and mutated Abl kinase domains except T315I. Autophosphorylation of the Abl kinase domain was analyzed for WT, Y253F, E255K, T315I, M351T, and H396P in the presence of escalating concentrations of AP23464 (0-320 nM) as previously described.23 Representative kinase assay gels from 1 of 2 independent experiments are shown. Abl blots demonstrate equal protein loading.
