Quantification of minimal residual leukemic disease in the DA1-3b/C3H mouse model. (A) Survival curves. C3H/Hej mice were vaccinated with irradiated DA1-3b leukemic cells that had been transduced with IL12 (DA1-3b/IL12), CD154 (DA1-3b/CD154), or control empty plasmid (DA1-3b/Zeo), and the mice were subsequently challenged with 10⁴ wild-type DA1-3b cells. (B-D) Number of residual leukemic cells in the spleen of mice treated as described in panel A at selected times after challenge. Horizontal mark represents the median.