Recovery of Foxp3 expression following BMT. (A) Changes in the Foxp3 mRNA expression in PBMCs from 34 patients following alloSCT. Three of 8 patients without GVHD were serially evaluated at 18, 24, 30, and 36 months after BMT, and patients with GVHD were assessed at time of first diagnosis; (•) indicates GVHD positive; (○), GVHD negative. Changes in Foxp3 expression over time were assessed in PBMCs from 2 representative patients with GVHD following BMT. Gray zone indicates mean ± SE of 10 healthy controls. Regression lines indicate correlation with GVHD (top: negative, bottom: positive). (B) Patient received transplant from a partially HLA-mismatched related donor developed severe continuous alloGVHD. (C) Patient developing autoGVHD after autoBMT and autoGVHD induction treatment. Relative expression of Foxp3 mRNA levels normalized against GAPDH was determined by real-time PCR.