Adenovirus-specific, serotype cross-reactive CTL line from donor AL. (A) At an E/T ratio of 20:1, the CTLs showed specific cytolytic activity against autologous adenovirus-infected targets in a standard chromium release assay with only slight or negligible killing of allogeneic, infected or uninfected targets or autologous fibroblasts alone. (B) Adenovirus-specific CTLs lysed autologous fibroblast targets transduced with Ad2, Ad4, Ad5, Ad6, Ad7, Ad8, and Ad11, but not allogeneic fibroblast targets, either alone or transduced with Ad5, Ad8, and Ad11.