CD83 expression on DCs treated with endothelin receptor antagonists. Results of flow cytometry analysis of DCs stained with CD83 antibodies. Representative histograms are shown from 5 independent experiments. DCs indicates immature DCs; DC + SA, mature DCs; DC + SA + ABT-627, mature DCs treated with ET_A receptor antagonist ABT-627 for 48 hours, leading to a significant lowering of CD83 expression in comparison with mature DCs (P < .001); and DC + SA + A-192621, mature DCs treated with ET_B receptor antagonist A-192621 for 48 hours, displaying a significant increase in CD83 expression in comparison with mature DCs (P = .014). Isotype control (black line) was anti-immunoglobulin G-conjugated to phycoerythrin. Cells stained with CD83 are indicated by gray lines. Numbers on the horizontal bar indicate the percentage of cells positive for CD83.