Surface plasmon resonance of mAb C11C1 binding to mouse kininogen present in mouse plasma. Diluted plasma prepared as outlined in “Materials and methods” was injected over a sensor chip to determine the ability of mAb C11C1 to bind to HK in mouse plasma. Control mAb (MOPC-21) was used to subtract nonspecific binding. Mouse plasma (solid line) and human plasma (dashed line) bound with similar kinetics. HK-deficient plasma (dotted line) did not bind. Each line represents averaged signals of 3 separate experiments.