Liver biopsy. Hematoxylin and eosin (A) and Prussian blue iron (B) stains of the patient's liver biopsy specimen at the time of diagnosis, demonstrating heavy (4+) hepatocellular iron deposition. Slides were viewed with a Nikon Eclipse E600 microscope equipped with a 40×/0.75 objective lens (Nikon, Melville, NY). An RT Slider SPOT 2.3.1. camera and SPOT advanced software (v.3.5.9; Diagnostic Instruments, Sterling Heights, MI) were used to capture images.