American Society of Hematology

Figure 5.

$Figure 5. Lack of class switching in B cells from bone marrow and peripheral blood of WM patients. RT-PCR of unfractionated mononuclear cells prepared from BM and peripheral blood of WM patients as described in “Patients and methods.” An equal amount of cDNA was used in each sample. Amplification was performed using CDR3R (patient specific) and respective CH1 primers. Negative controls of PCR reaction without cDNA or with cDNA from BM or blood of healthy controls (n = 2), or other WM patients (n = 8) did not yield any bands.$

Lack of class switching in B cells from bone marrow and peripheral blood of WM patients. RT-PCR of unfractionated mononuclear cells prepared from BM and peripheral blood of WM patients as described in “Patients and methods.” An equal amount of cDNA was used in each sample. Amplification was performed using CDR3R (patient specific) and respective C_H1 primers. Negative controls of PCR reaction without cDNA or with cDNA from BM or blood of healthy controls (n = 2), or other WM patients (n = 8) did not yield any bands.

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