American Society of Hematology

Figure 7.

$Figure 7. Effect of Dp44mT (1 μM) in cultured M109 cells on the holocytochrome c (h-cytc) levels in cytosolic and stromal-mitochondrial membrane (SMM) fractions and mitochondrial protein levels of Bcl-2 and Bax. (A) Cytosolic and SMM fractions of M109 cells were separated and subjected to Western blotting with anti–h-cytc antibody. The blot was reprobed with an anti–β-actin antibody to ensure equal protein loading. Densitometric analyses are shown beneath the blots; expression is normalized to β-actin. (B) Protein levels of Bcl-2 and Bax were determined through Western blotting using the SMM fraction of M109 cells after incubation with Dp44mT for 0 to 48 hours. Anti–β-actin antibody was used to ensure equal protein loading. Densitometric analysis is shown beneath each blot, where expression is normalized to β-actin. Results in panels A and B are representative of 3 experiments.$

Effect of Dp44mT (1 μM) in cultured M109 cells on the holocytochrome c (h-cytc) levels in cytosolic and stromal-mitochondrial membrane (SMM) fractions and mitochondrial protein levels of Bcl-2 and Bax. (A) Cytosolic and SMM fractions of M109 cells were separated and subjected to Western blotting with anti–h-cytc antibody. The blot was reprobed with an anti–β-actin antibody to ensure equal protein loading. Densitometric analyses are shown beneath the blots; expression is normalized to β-actin. (B) Protein levels of Bcl-2 and Bax were determined through Western blotting using the SMM fraction of M109 cells after incubation with Dp44mT for 0 to 48 hours. Anti–β-actin antibody was used to ensure equal protein loading. Densitometric analysis is shown beneath each blot, where expression is normalized to β-actin. Results in panels A and B are representative of 3 experiments.

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