The cDCs induce primary-like CD8+T-cell responses in mice that have been previously immunized with immature or CpG-activated pDCs. The 129sv mice were injected with purified immature or CpG-activated cDCs or pDCs loaded or not with OVA257-264 peptide (1st injection). Three weeks later, mice received a second injection of purified cDCs loaded or not with OVA257-264 peptide (2nd injection). Seven days after the second injection, the frequency of peptide-specific, IFN-γ–producing cells (A) and the IFN-γ concentration in response to OVA257-264 peptide (B) were determined. Each symbol corresponds to an individual mouse. Horizontal bars represent medians. The cumulative results of 4 independent experiments are shown. Statistical analysis compared the responses induced by OVA-loaded cDCs to the responses induced by the other DC groups. The same degree of statistical significance was obtained for the results of the ELISPOT and ELISA experiments.
