Figure 5.
E2 pretreatment enhance the T-cell stimulatory capacity of mature DCs. Immature DCs were treated with BC, E2 (20 μg/mL), or medium only for 24 hours. To mature the cells, the pretreated iDCs were then stimulated with LPS (1 μg/mL) for 24 hours. Different concentrations of immature (A) or mature (B) DCs (0-50 000) were cultured in round-bottomed 96-well plates in triplicates with 50 000 allogeneic CD4+ T cells for 5 days in RPMI with 10% FCS. T-cell proliferation was assessed. All determinants were performed in triplicate and measured as the mean cpm ± SD. The figure shows 1 representative experiment of 4.
