Figure 4.
Figure 4. Lymphoma cell microvilli are dynamic and actin dependent. (A) Phase-contrast micrograph of a fixed 300.19 cell. (B) Rhodamine fluorescence of same 300.19 cell stained with rhodamine-phalloidin. (C) Time course of decrease in cellular rhodamine-phalloidin after treating 300.19 cells with 1 mm LatA or with dimethyl sulfoxide (DMSO) carrier. (D) SEM of 300.19 cell 2 minutes after treatment with 1 μM LatA. (E-G) SEMs of 300.19 cells before treatment (E), after 10 minutes in 1 μM LatA (F), and 30 minutes after LatA wash out (G). Scale bars are 2 μm. Original magnification × 1000 (A-B) and × 10 000 (D).

Lymphoma cell microvilli are dynamic and actin dependent. (A) Phase-contrast micrograph of a fixed 300.19 cell. (B) Rhodamine fluorescence of same 300.19 cell stained with rhodamine-phalloidin. (C) Time course of decrease in cellular rhodamine-phalloidin after treating 300.19 cells with 1 mm LatA or with dimethyl sulfoxide (DMSO) carrier. (D) SEM of 300.19 cell 2 minutes after treatment with 1 μM LatA. (E-G) SEMs of 300.19 cells before treatment (E), after 10 minutes in 1 μM LatA (F), and 30 minutes after LatA wash out (G). Scale bars are 2 μm. Original magnification × 1000 (A-B) and × 10 000 (D).

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