Rhesus LCV-positive B-cell lymphoma from Mm256-96. (A-B) Hematoxylin and eosin–stained section of submandibular tumor mass from necropsy on day 155 after intravenous inoculation of autologous rhesus LCV-immortalized B cells. Original magnification × 40 (A) and × 200 (B). (C-G) Immunohistochemical staining of tumor section for CD20 (C), CD3 (D), bcl-2 (E), Ki-67 (F), and EBNA-2 (G). Original magnification × 200. (H) In situ hybridization of tumor section for EBER expression. Original magnification × 200. (I-J) Fluorescent in situ hybridization with a rhesus LCV DNA probe on touch preps prepared from the (I) tumor section or (J) EBV-negative BJAB B lymphoma cells. Original magnification × 400.