Analyses of endothelial-specific protein-DNA complex formation with sequences +155 to +184. (A) Represents the oligonucleotide probes that collectively span sequences +121 to +247 region of the VWF gene. Oligonucleotide probes (bracketed) are designated HH1 to HH7, with beginning and ending nucleotides shown. (B) Gel mobility assays were performed with use of HH1, HH2, and HH3 double-stranded oligonucleotide probes (10 000 cpm) and nuclear extracts (5 μg) prepared from bovine aortic endothelial (BAE) and bovine aortic smooth muscle (BSM) cells. (C-D) Gel mobility assays were performed with use of HH2 probe (10 000 cpm) corresponding to sequences +155 to +184 and nuclear extracts (5 μg) prepared from (C) HUVECs and HeLa cells and (D) HUVEC (HUV) and HEK293 (HEK) cells. The absence of competitor is represented by “-”. S (double-stranded unlabeled oligonucleotide +155 to +184) and N (double-stranded unlabeled oligonucleotide -490 to -460) represent specific and nonspecific oligonucleotide competitors. The arrows represent the endothelial specific complex in each panel.