Family tree and analysis of WASP expression. (A) Simplified pedigree of the study family. Solid squares represent affected men; diagonal lines indicate deceased patients. Carrier status in female patients is indicated by a central dot. (B) Flow cytometric analysis of WASP. PBMCs were fixed, permeabilized, and stained with anti-WASP monoclonal antibody (mAb) (solid histogram) or a control antibody (open histogram), followed by phycoerythrin (PE)–conjugated goat antimouse antibody. Shown are the results of WASP expression in lymphocytes and monocytes from a healthy control and from patients III-1, III-2, III-6, and IV-1.