Thrombin causes PKCζ relocalization in ECs. (A) Cells were stained for the 2 atypical isoforms of PKC, ζ and λ (i,iii), and for the junctional protein, VE-cadherin (ii,iv). (B) EC monolayers were stained to demonstrate changes in PKCλ (i) and PKCζ (iii) localization following treatment with thrombin (0.2 U/mL). Cells also were stained for VE-cadherin (ii,iv) to demonstrate changes in cell-cell interactions following thrombin stimulation. (C) ECs were stained for PKCα (i-ii) and PKCϵ (iii-iv). Treatment of cells with thrombin does not alter PKCα (ii) and PKCϵ (iv) localization. (D) Thrombin causes PKCζ redistribution to membranes. PKCζ immunoblot of cytosolic and membrane fractions of PKCζ-infected HUVE cells, fractioned following incubation with (+) or without (-) thrombin (0.2 U/mL) for 15 minutes.