Direct DNA sequencing and SSCP analysis ofAML1. (A) The sequences depict mutations identified in exon 4 of AML1 and the corresponding wild-type (WT) sequences. The first sequence shows a deletion of 1 nucleotide identified in patient 98, the second sequence shows a insertion of 14 nucleotides identified in patient 160, and the final sequence shows a single base substitution in patient 165. (B) Representative PCR/SSCP bands (top) and RT-PCR/SSCP bands (bottom) of exon 4. Lanes 1 and 5 are healthy controls and lanes 2 to 4 are patient nos. 98, 160, and 165.