FACS analysis of hematopoietic cells from the TG(zpu.1:EGFP)^df5 adult fish. Cells from kidney (A-B), spleen (C-D), and blood (E-F) from adult transgenic zebrafish were isolated and analyzed by FACS for total cellular subfractions by light-scatter gating (A,C,E) and the same subfractions of EGFP-positive cells (B,D,F). Gated populations are as follows: erythrocytes (red), lymphocytes (blue), granulocytes and monocytes (green), and blood cell precursors (purple). Cell size is represented by forward scatter (FSC; abscissa), and granularity is represented by side scatter (SSC; ordinate). Mean percentage of cells is indicated for each gated subpopulation.