Fluorescent images of TG(zpu.1:EGFP)^df5 embryos at 22 hpf. (A) Lateral view of GFP-positive cells. (B) Magnified lateral view of the posterior tail in panel A. (C) Dorsal view of the anterior head region. The white arrows indicate zpu.1-expressing myeloid cells migrating from the anterolateral mesoderm over the yolk; and the red arrows, zpu.1 cells in the ICM. (D) Confocal image of 2-color in situ hybridization of cells migrating over the anterior yolk at 22 hpf using a zpu.1 RNA probe (red) and an antibody to EGFP (green). Yellow indicates coexpression. Original magnification × 40 (A, D); × 100 (B-C).