![Figure 5. Strength and persistence of stimulation with intact E coli modulate MoDC migration and cytokine secretion. MoDCs were stimulated with intact E.coli as indicated. One half of the cultures were washed twice 3 hours after addition of E coli and then cultured in RPMI/FCS until 48 hours (mean ± SEM of 8 experiments are shown [n = 4 for E coli 1:1000]). (A) Migration toward CCL21 and (C) IL-12p70 secretion or (E) IL-6 secretion of MoDCs activated with varying concentrations of intact E coli. (*P < .05; **P < .01 as compared with E coli 1:1). Effect of washing MoDCs following 3 hours of activation with varying concentrations of live E coli on (B) migration toward CCL21 and (D) IL-12p70 secretion or (F) IL-6 secretion. Cultures were continued in RPMI/FCS for a total of 48 hours. Mean ± SEM of 8 experiments are shown (n = 4 for E coli 1:1000), *P < .05; **P < .01 compared with unwashed MoDCs (normalized to 1).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/4/10.1182_blood-2003-12-4146/6/zh80160465140005.jpeg?Expires=1722835490&Signature=aS9Xytnz0LJAWCACdehRXHaweOjTPajbW-FjIgs4uoAzqGB3y-CJWzPLdWFSH6k~McwJooh2ZOWKv9VfSklVhPD~u4mgRkL7QFW6QtrGUcDQDmFtLICs7E-1FMNZl5~6td3DSK9ett01HXekIiNUswxpPt91sdj-Nh-04WQHVmIqWIuN5RR9NMfXgQdqDKmyMjEsrK6KMm8Sa1zkEQZUZMe6cMzEjov6u5QejqVJy-0zq3D4uvQykqGuq81emndyYgJFOCGQjREz4hah3sEqDzK4AAfAPNFB2hpwfB6AVxl2DAvE1n-S75f0irW3N9XuVV7AozEaBogqoRCsHhtr6w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Strength and persistence of stimulation with intactE colimodulateMoDC migration and cytokine secretion. MoDCs were stimulated with intact E.coli as indicated. One half of the cultures were washed twice 3 hours after addition of Ecoli and then cultured in RPMI/FCS until 48 hours (mean ± SEM of 8 experiments are shown [n = 4 for E coli 1:1000]). (A) Migration toward CCL21 and (C) IL-12p70 secretion or (E) IL-6 secretion of MoDCs activated with varying concentrations of intact E coli. (*P < .05; **P < .01 as compared with E coli 1:1). Effect of washing MoDCs following 3 hours of activation with varying concentrations of live E coli on (B) migration toward CCL21 and (D) IL-12p70 secretion or (F) IL-6 secretion. Cultures were continued in RPMI/FCS for a total of 48 hours. Mean ± SEM of 8 experiments are shown (n = 4 for E coli 1:1000), *P < .05; **P < .01 compared with unwashed MoDCs (normalized to 1).
