![Figure 1. The expression of ephrinB2 and EphB4 proteins in pyogenic granuloma and cell cultures. (A) A representative double staining from specimens of pyogenic granuloma, which is a hyperreactive inflammatory lesion. EphB4 was detected with peroxidase-AEC (3-amino-9-ethylcarbazole) and appeared red (red arrow). Positive staining for ephrinB2 was dark blue, which was detected by alkaline phosphatase–NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indoyl phosphate p-toluidine salt). Most of the ephrinB2-positive cells were macrophage-like cells (white arrowhead), while EphB4-positive cells were in small venules. Original magnification × 400. Scale bar equals 20 μm. (B) Western blots for ephrinB2 and EphB4 in 5 human primary cell cultures, including 4 endothelial cells (HUAECs, HUVECs, HIAECs, HSVECs) and 1 fibroblast (human gingival fibroblast [HGF]), and 1 monoblastoid cell line (U937) activated with 20 ng/mL PMA. [Beta]-Actin was an internal control.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/4/10.1182_blood-2003-09-3334/6/zh80160465120001.jpeg?Expires=1722799274&Signature=qIMatm-nfnyeZAE6oGJIYr3RfOCtR9B1BmdjZL~vbGrEWE9xUBWB96Bk0IpZqMF0eG~wf17GG4Ma05NLJWQ-y1Lv5Hs1W4XQNuay66ZOzAnStYGsu8jrxfXHfAPnAQ~eqk8J1vE3L~4ohQ8nQYOf3UtUTYFEwnRdL3NhgcqJiwbXuzxX1uHGZli12pkwC5PcEiyNH-sFZb4d0sojZIV~IdyKtlxjP5q1bd1xWtYXJpxA6rZaIQkc0UXad6AIdBl0RrIkrSvPOA0QwIcG9H6vin315WDjBc1~UbN6JEL0OfxoFm6-haDVPLvpuvSZuN1VNdj6iVm4lLYNlR9tvuUsew__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The expression of ephrinB2 and EphB4 proteins in pyogenic granuloma and cell cultures. (A) A representative double staining from specimens of pyogenic granuloma, which is a hyperreactive inflammatory lesion. EphB4 was detected with peroxidase-AEC (3-amino-9-ethylcarbazole) and appeared red (red arrow). Positive staining for ephrinB2 was dark blue, which was detected by alkaline phosphatase–NBT/BCIP (nitroblue tetrazolium/5-bromo-4-chloro-3-indoyl phosphate p-toluidine salt). Most of the ephrinB2-positive cells were macrophage-like cells (white arrowhead), while EphB4-positive cells were in small venules. Original magnification × 400. Scale bar equals 20 μm. (B) Western blots for ephrinB2 and EphB4 in 5 human primary cell cultures, including 4 endothelial cells (HUAECs, HUVECs, HIAECs, HSVECs) and 1 fibroblast (human gingival fibroblast [HGF]), and 1 monoblastoid cell line (U937) activated with 20 ng/mL PMA. [Beta]-Actin was an internal control.
