Figure 5.
![Figure 5. Mechanism of septic RBC-mediated vasodilation. (A) Vessel cGMP content in rat thoracic aorta segments increased following incubation with septic RBCs (0.01 [1%] hematocrit). (B) Relaxation effects of septic RBCs were inhibited on cell lysis. (C) Effects of lysis, GSH (100 μM), C-PTIO (100 μM), ascorbate (100 μM), or apyrase (5 U/mL) on septic RBC– (0.01 [1%] hematocrit) mediated relaxation. For RBC lysis, amounts of RBC lysates equivalent to 0.01 (1%) hematocrit intact RBCs were added. Data represent mean ± SEM (n = 3). *P ≤ .05 versus LPS RBCs.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/5/10.1182_blood-2004-03-0880/5/zh80170466140005.jpeg?Expires=1724273073&Signature=1Vn6atchN4AykvM5EncYQ1oeurH43tMww3foRkGScKg7M8dTjG7rtWqF1zqMi-MYl3ZGCnAQRQcP1TjxEQMk~ICodgjpAgPvUovz~TCeIPEa4m6nMsQafy8dpnZpKDG~iCjRLCQswIbhL5hWsEj2rYPLbXzw7i74kWc2uyH6PT6a3AhIy2ToXQGhg8aQk8hbE~ErMSO68irrowCLRr7172zo8~0Clm4Gab5L-9KoXuzxE1HUv2yIm-4QrCLZpbL1UV0quZe0Aym8a8pi4J-vdcszmN5QsTH5QwBOxg~w-Ny4GkwCSOb6hYaShshU0qL51bs0C7xtUCneijHw1GbsZQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mechanism of septic RBC-mediated vasodilation. (A) Vessel cGMP content in rat thoracic aorta segments increased following incubation with septic RBCs (0.01 [1%] hematocrit). (B) Relaxation effects of septic RBCs were inhibited on cell lysis. (C) Effects of lysis, GSH (100 μM), C-PTIO (100 μM), ascorbate (100 μM), or apyrase (5 U/mL) on septic RBC– (0.01 [1%] hematocrit) mediated relaxation. For RBC lysis, amounts of RBC lysates equivalent to 0.01 (1%) hematocrit intact RBCs were added. Data represent mean ± SEM (n = 3). *P ≤ .05 versus LPS RBCs.
