Partial dysfunction in XID macrophages. (A) ROI responses of PEC macrophages from WT (white symbols) or XID (black symbols) mice to varying concentrations (μg/mL) of LPS over time as indicated (mean ± standard error [SE] of triplicate cultures). (B) ROI responses of PEC macrophages from WT or XID mice to PMA (30 ng/mL) over time (mean ± SE of triplicate cultures). (C) Phagocytosis of fluorescein-labeled, heat-killed bacteria by PEC macrophages from WT (thin lines) or XID (thick lines) mice, shown as histograms of fluorescein intensity in gated CD11b⁺ cells either not exposed to bacteria (-), or exposed to them at 4°C (4 C) or at 37°C (37 C) for 1 hour. Percentages of CD11b⁺ cells with associated bacterial fluorescence are indicated. (D) Time course of phagocytosis of fluorescein-labeled, heat-killed bacteria by PEC macrophages from WT or XID mice in gated CD11b⁺ cells exposed to labeled bacteria at 37°C for varying times as indicated. (E) Time course of motility of PEC macrophages from WT or XID mice in vitro. (F) Death of PEC macrophages from WT or XID mice upon exposure to medium alone (-), or the IFNγ (50 IU/mL) along with either low-dose LPS (3 μg/mL) or high-dose LPS (30 μg/mL). Death was scored over time flow cytometrically by PI staining of CD11b⁺ cells.